Soybean variety OT21325816

ABSTRACT

A novel soybean variety, designated OT21325816 is provided. Also provided are the seeds of soybean variety OT21325816, cells from soybean variety OT21325816, plants of soybean OT21325816, and plant parts of soybean variety OT21325816. Methods provided include producing a soybean plant by crossing soybean variety OT21325816 with another soybean plant, methods for introgressing a transgenic trait, a mutant trait, and/or a native trait into soybean variety OT21325816, methods for producing other soybean varieties or plant parts derived from soybean variety OT21325816, and methods of characterizing soybean variety OT21325816. Soybean seed, cells, plants, germplasm, breeding lines, varieties, and plant parts produced by these methods and/or derived from soybean variety OT21325816 are further provided.

FIELD OF THE DISCLOSURE

This invention relates generally to the field of soybean breeding. Inparticular, the invention relates to a soybean variety designatedOT21325816 that includes plants, plant parts, and seeds of soybeanvariety OT21325816. Methods for producing soybean plants by crossingsoybean variety OT21325816 with itself or any different soybean plantare an integral part of this invention as are the resultant soybeanplants, including the plant parts and seeds. This invention furtherrelates to methods for producing OT21325816-derived soybean plants andto methods for regenerating such plants from tissue cultures ofregenerable cells as well as the plants obtained therefrom. Methods forproducing a soybean plant containing in its genetic material one or moretransgenes and to the transgenic soybean plants produced by that methodare also a part of this invention.

BACKGROUND

Soybean (Glycine max) is a major grain crop valued for the high levelsof oil and protein found in soybean seed. Soybean breeding has resultedin significant improvements in yield potential, stability of yield,adaptation of the species to mechanical harvest, and yield protectionthrough improved disease resistance.

Due to the nature of plant science agriculture, broadly defined as amanipulation of available plant resources to meet the needs of thegrowing human population, the environment in which plants are grown foragricultural production continuously offers new obstacles toagricultural production. Each new variety, or cultivar, released toagricultural production is selected for the purpose of increasing yieldresulting from increased disease resistance to prevalent diseases, orfrom direct or indirect improvement in yield potential or efficiency ofproduction. Development of stable, high yielding varieties with superiorcharacteristics is an ongoing goal of soybean breeders.

There is a need in the art for a novel, superior soybean variety andsoybean seed.

SUMMARY

In one aspect, the present invention provides a soybean seed designatedOT21325816.

In another aspect, the present invention provides a soybean plant, or apart thereof, produced by growing seed designated OT21325816, or asoybean plant having all the physiological and morphologicalcharacteristics of the soybean plant when grown in the sameenvironmental conditions, or a pollen grain, an ovule, a protoplast, acell, an embryo, a cotyledon, a hypocotyl, a meristem, a root, a pistil,an anther, a flower, a stem, a pod, a leaf, or a petiole of a soybeanplant according to the present invention.

In yet another aspect, the present invention provides a tissue cultureof protoplasts or regenerable cells from a plant, or parts thereof,produced by growing seed designated OT21325816, and a soybean plantregenerated from the tissue culture. In some embodiments, theprotoplasts or regenerable cells are derived from embryo, meristematiccell, leaf, pollen, ovule, cotyledon, hypocotyl, root, root tip, anther,pistil, pod, flower, shoot or stalk.

In still another aspect, the present invention provides a method forproducing a soybean seed, and soybean seed produced by the method, aswell as plants grown from seed produced by the method are provided. Themethod comprises crossing soybean plants and harvesting the resultantseed, wherein at least one soybean plant is the soybean varietyOT21325816 of the present invention.

In another aspect, a method for producing a soybean varietyOT21325816-derived soybean plant, and soybean variety OT21325816-derivedsoybean plants, or parts thereof, produced by the methods are provided.The method comprises crossing a soybean variety OT21325816 plant of thepresent invention with a second soybean plant to yield F1 hybrid progenysoybean seed and growing the progeny seed to yield an F1 soybean varietyOT21325816-derived soybean plant. In some embodiments, the methodfurther comprises crossing the soybean variety OT21325816-derivedsoybean plant with itself or another soybean plant to yield anadditional soybean variety OT21325816-derived soybean progeny seed andgrowing this progeny soybean seed to yield additional soybean varietyOT21325816-derived soybean plants. In some embodiments, these last twosteps are repeated at least one time to generate additional soybeanvariety OT21325816-derived soybean plants.

In yet another aspect, a method for producing a plant of soybean varietyOT21325816 comprising an added desired trait, and plants produced by themethod, are provided. In some embodiments, the method comprisesintroducing at least one transgene or locus, such as a geneticmodification, conferring the desired trait into the soybean varietyOT21325816 plant. In certain embodiments, the desired trait is selectedfrom the group consisting of male sterility, site-specificrecombination, abiotic stress tolerance, herbicide tolerance, insect orpest resistance, disease resistance, fungal resistance, modified fattyacid metabolism, modified protein metabolism, and modified carbohydratemetabolism. In other embodiments, the desired trait is herbicidetolerance and the tolerance is conferred to one or more herbicidesselected from the group consisting of glyphosate, phenoxyacetate auxins(such as 2,4-diclorophenoxyacetic acid (2,4-D)), pyridyloxyacetateauxins (such as fluroxypyr and triclopyr), phenoxyproprionate auxins(such as MCPA and dichloprop), phenoxybutanoate auxins (such as 2,4-DB),sulfonylurea, imidazalinone, dicamba, glufosinate, cyclohexone,triazine, and benzonitrile. In still other embodiments, the desiredtrait is insect resistance and the transgene encodes a Bacillusthuringiensis (Bt) endotoxin.

Another aspect of the current invention is a soybean plant furthercomprising a locus conversion, such as a single locus conversion. In oneembodiment, the soybean plant is defined as comprising the locusconversion and otherwise capable of expressing all of the morphologicaland physiological characteristics of the soybean variety OT21325816. Byall or essentially all of the morphological and physiologicalcharacteristics, it is meant that all of the characteristics of a plantare recovered that are otherwise present when compared in the sameenvironment, other than an occasional variant trait that might ariseduring backcrossing or direct introduction of a transgene or targetedgenetic modification. In particular embodiments of the invention, thelocus conversion may comprise a transgenic gene which has beenintroduced by genetic transformation into the soybean variety OT21325816or a progenitor thereof. In still other embodiments of the invention,the locus conversion may comprise a dominant or recessive allele. Thelocus conversion may confer potentially any trait upon the single locusconverted plant, including herbicide resistance, insect resistance,resistance to bacterial, fungal, or viral disease, male fertility orsterility, and improved nutritional quality.

In still another aspect, a method of producing a progeny soybean varietyderived from variety OT21325816 comprising a desired trait, and plantsproduced by the method, are provided. In some embodiments, the methodcomprises crossing a soybean variety OT21325816 plant of the presentinvention with a plant of another soybean variety that comprises adesired trait to produce F1 progeny plants, selecting one or more F1progeny plants that have the desired trait to produce selected progenyplants, crossing the selected progeny plants with the OT21325816 plantsto produce backcross progeny plants, selecting for backcross progenyplants that have the desired trait and physiological and morphologicalcharacteristics of soybean variety OT21325816 to produce selectedbackcross progeny plants, and repeating the last two steps a sufficientnumber of times in succession to produce selected second or higherbackcross progeny plants that comprise the desired trait and thephysiological and morphological characteristics of soybean varietyOT21325816 when grown in the same environmental conditions. In someembodiments, the last two steps are repeated three or more times insuccession to produce selected fourth or higher backcross progenyplants. In some embodiments, the desired trait is selected from thegroup consisting of male sterility, herbicide tolerance, insectresistance, modified fatty acid metabolism, modified carbohydratemetabolism, and resistance to bacterial disease, fungal disease or viraldisease.

In yet another aspect, a method of producing a commodity plant productis provided, which comprises obtaining a plant of the present invention,or a part thereof, and producing the commodity plant product therefrom.In some embodiments, the commodity plant product is protein concentrate,protein isolate, soybean hulls, meal, flour or oil.

DETAILED DESCRIPTION

In the claims, descriptions, and tables that follow, numerous terms areused and are defined as follows:

Aerial Web Blight caused by the fungus, Rhizoctonia solani, is visuallyscored from 1 to 9 based on the severity of fungal spots on vegetativetissue. A score of 1 indicates the most tolerant (no symptoms) and ascore of 9 indicates the most susceptible.

Brown Stem Rot (BSR) caused by the fungus, Phialophora gregata, isvisually scored from 1 to 9 based on the severity of interveinal leafchlorosis (yellowing) and necrosis of stems. A score of 1 indicates themost resistance (no symptoms) and a score of 9 indicates the mostsusceptible.

Canopy width is measured at the widest point of the soybean plant.Visually scored from 1 to 9, where 1=very narrow canopy and 9=extremelybushy canopy.

Charcoal Rot Drought Complex caused by the fungus, Macrophominaphaseolina, is a sandy-soil, mid-summer disease distinguished by grayspeckling within the lower stems of soybean plants.

Cotyledon is a type of seed leaf. The cotyledon contains the foodstorage tissues of the seed. Cotyledon color can be measured as acharacteristic of a variety.

Flower color: Modern soybeans are characterized by two major flowercolors, purple or white. Some varieties are heterogeneous for flowercolor whereby some plants have purple flowers and some have white.

Frogeye Leaf Spot is caused by the fungus, Cercospora sojina. The fungussurvives as mycelium in infected seeds and in infested debris. Withadequate moisture new leaves become infected as they develop until allthe leaves are infected. Yield losses may be up to 15% in severeinfected fields. Frog Eye Leaf Spot (FELSR) rating is a field rating (1to 9 scale) based on the percentage of leaf area affected. The scale is1 to 9 where 1=no leaf symptoms and 9=severe leaf symptoms. One is thebest rating. To test varieties for Frog Eye Leaf Spot a disease nurseryis artificially inoculated with spores. The ratings are done when theplants have reached the R5 to R6 growth stage. Visual calibration isdone with leaf photos of different frogeye severity ratings.

Growth habit refers to stem termination in soybeans and the resultantdifferences in flower production. “Indeterminate” varieties continue togrow during the reproductive phase, producing new branches and nodesafter flowering is well underway. “Determinate” varieties tend to delaythe onset of flowering somewhat, and limit new node and branchdevelopment after flowering has been initiated. “Semi-determinate”varieties continue to produce new vegetative growth during thereproductive phase but growth terminates more quickly than inindeterminate varieties.

Hilum refers to the point of attachment of soybean seed to maternaltissue.

Hilum color in modern soybeans may be black, brown, yellow, gray, buff,or imperfect black.

Iron-Deficiency Chlorosis (IDC) results when soybeans lack adequateiron. A visual score taken 25 to 30 days after planting is used to rateiron-deficiency chlorosis. A score of 1 indicates no stunting of theplants or chlorosis of the leaves, and a score of 9 indicates the plantsare dead or dying as a result of iron-deficiency chlorosis. A score of4.5 means plants have intermediate health with some leaf chlorosis.

Leaflet shape: The leaflet may be broad or narrow and may be lanceolate,ovate or oval in shape.

Locus Conversion: Refers to seeds, plants, and/or parts thereofdeveloped by backcrossing or genetic transformation wherein essentiallyall of the desired morphological and physiological characteristics of avariety are recovered in addition to at least one locus which has beentransferred into the variety by introgression, backcrossing or genetictransformation. The locus can be a native locus, a transgenic locus, ora combination thereof.

Lodging relates to the stature of the plant relative to the ground.Lodging resistance is rated on a scale of 1 to 5. A score of 1 is givento an erect plant (lodging resistant). A score of 3 is given to a plantthat is leaning at a 45-degree angle relative to the ground. A score of5 indicates a plant lying on the ground.

Maturity date is the date when 95% of pods have turned color from greencolor to their mature brown or tan color. The maturity date is countedin days and is calculated from January 1.

Maturity group refers to an industry division of groups of varietiesbased on the zones in which the varieties are adapted. Soybeans maturedifferentially in response to day-length and thus to latitude wheregrown. In the soybean production areas of the United States, forexample, the northernmost production region of northern Minnesota isplanted to soybeans that mature under very long day-lengths during earlysummer. In the southernmost production regions of the Southeast,soybeans that mature from the influence of short day-length during earlysummer are grown. Those adapted to northern day-lengths are classifiedas early-maturing, those adapted to the southern regions are classifiedas late-maturing. Maturity groups include very long day length varieties(000, 00, 0) and extend to very short day length varieties (VII, VIII,IX, X). For example, maturity group I soybean varieties are typicallygrown in southern Minnesota, whereas maturity group IV soybean varietiesare typically group in southern Illinois.

Oil Percent refers to percentage of total oil in seed.

Phytophthora “Root Rot” tolerance caused by the fungus, Phytophthoramegasperma var. sojae, is rated on a visual scale of 1 to 9, with ascore of 1 being the most tolerant and a score of 9 s being the mostsusceptible to Phytophthora. The visual score is based on the amount ofdisease-induced stunting of above-ground growth and is taken at harvest.

Plant includes plant cells, plant protoplasts, plant cell tissuecultures from which soybean plants can be regenerated, plant calli,plant clumps, and plant cells that are intact in plants or partsthereof. “Plant part” includes, but is not limited to, embryos,protoplasts, cells, pollen, ovules, cotyledons, hypocotyls, meristems,roots, pistils, anthers, flowers, stems, leaves, pods, petioles, and thelike.

Plant height is measured from the top of soil to top node of the plantin any convenient unit of length (i.e., inches, centimeters, etc.). Forthe data presented herein, plant height was measured just prior toharvest and is expressed in centimeters.

Pod wall color refers to the color of the mature pod wall, as distinctfrom the color of the pubescence, and in modern soybeans, may be brownor tan.

Protein Percent refers to the percentage of crude protein in the driedseed.

Pubescence relates to the plant trichomes or hairs found on the stems,leaves and pods of soybeans.

Pubescence color in modern soybeans may be tawny, gray or light tawny.

Relative maturity, within maturity groups, is a more precise maturityassignment that subdivides each maturity group into tenths. For example,a relative maturity of 3.3 is assigned to a late early maturity groupIII soybean variety.

Root-knot nematode resistance is based on a 45-day greenhouse screen ofsoybean roots inoculated with eggs and juveniles of Meloidogyne spp.Rating scale is based upon female reproduction index on a susceptiblecheck set determined by number of galls present. Rating scale is 1 to 9with 1 being most resistant and 9 being most susceptible.

Roundup Ready 2 tolerance refers to the transgenic soybean eventMON89788 from Monsanto Company, as identified in the USDA petitionextension No 06-1′780-01p, which imparts glyphosate tolerance to theplant.

Sclerotinia Stem Rot, also referred to as “white mold”, is caused by thesoil-borne fungus, Sclerotinia sclerotiorum. Plants are infected viadischarged ascospores that successfully germinate and infect throughsoybean structures such as flower petals. Colonization of stem, pod, andleaf tissue ultimately results in loss of yield potential. Cultivars arerated based on prevalence and severity of a 1 to 9 scale, with 1 beingno infection and 9 being severe infection.

Seed coat color refers to the color of the seed coat, and in modernsoybeans may be yellow, green, brown or black.

Seed coat luster refers to the luster of the seed coat, and may be dullor shiny.

Seed coat peroxidase activity is defined by a chemical taxonomictechnique to separate varieties based on the presence or absence of theperoxidase enzyme in the seed coat. There are two types of soybeanvarieties, those having high peroxidase activity and those having lowperoxidase activity. Ratings are HIGH or LOW for peroxidase enzymeactivity.

Seed size is measured by seed number per pound of seed. Seed size is aheritable trait but is influenced by environment, and as such, is oftenpresented as a comparison to another variety.

Shattering refers to pod dehiscence prior to harvest resulting in a lossof mechanically harvestable seed. Pod dehiscence involves seeds fallingfrom the pods to the soil. This is visually scored with a 1 to 9 scalecomparing all genotypes within a given test. A score of 1 means podshave not opened and no seeds have fallen out. A score of 5 indicatesapproximately 50% of the pods have opened, with seeds falling to theground and a score of 9 indicates 100% of the pods are opened.

Soybean Cyst Nematode (SCN) resistance is based on a comparison ofreproduction rates of Heterodera glycines to a known susceptible varietyas described by Schmitt et al. (Crop Sci. 32:275-277, 1992), which isincorporated by reference herein. A variety with a 0% to 10%reproductive rate compared to a known susceptible variety is classifiedas resistant (R); a variety with an 11% to 30% reproductive ratecompared to a known susceptible variety is classified as moderatelyresistant (MR); a variety with an 31% to 59% reproductive rate comparedto a known susceptible variety is classified as moderately susceptible(MS).

Soybean emergence scores, also referred to simply as “Emergence,” ratethe ability of the seedlings to emerge from the soil. A visual score of1 to 9, taken from emergence to V3, is used whereby a score of 1 to 3indicates excellent emergence vigor and early growth, an intermediatescore of 5 indicates average ratings, and a score of 7 to 9 indicates avery poor emergence vigor and early growth.

Stem Canker is caused by the fungus, Diaporthe phaseolorum, andtolerance is scored 1 to 9, with 1 being most tolerant and 9 being mostsusceptible, based on the number of lesions.

Sudden Death Syndrome (SDS) is caused by slow-growing strains of thefungus, Fursarium solani. The disease is a mid to late season,soil-borne disease in soybean fields. Yield losses may be total orsevere in infected fields. The SDS rating is an opportunistic fieldrating based on leaf area affected. The scale used for these tests is 1to 9. A score of 1 indicates the most tolerant (least symptoms) and ascore of 9 indicates the most susceptible.

Sulfonylurea Tolerance is a herbicide-tolerance trait that improvessoybean tolerance to ALS (acetolactate synthase) inhibitor herbicides.Sulfonylurea Tolerant soybeans carry a modified ALS gene, which enhancesthe variety's natural tolerance to sulfonylurea.

Yield refers to the yield of seed harvested from a soybean crop. Yielddata presented herein is expressed as bushels of seed/acre and is theactual yield of the grain at harvest.

Soybean Variety OT21325816

The present invention provides plants, seeds, plant parts, andderivatives thereof of the soybean variety OT21325816, characterized bymolecular and physiological data obtained from the representative sampleof said variety deposited with the American Type Culture Collection(ATCC). The present invention further provides methods for producingsoybean variety OT21325816 and methods for breeding with soybean varietyOT21325816 to produce novel derived soybean varieties.

Soybean variety OT21325816 has superior characteristics and wasdeveloped from crossing elite soybean varieties. Some of the criteriaused to select the variety in various generations included seed yield,lodging resistance, emergence, disease resistance and tolerance,herbicide tolerance, maturity, late season plant intactness, plantheight, and shattering resistance.

Soybean variety OT21325816 has been judged to have uniformity andstability of its morphological and other characteristics. The varietycan be reproduced by planting and growing seeds of the variety underself-pollinating or sib-pollinating conditions, as is known to those ofskill in the agricultural arts. Soybean variety OT21325816 shows novariants other than what would normally be expected due to environmentor that would occur for almost any characteristic during the course ofrepeated sexual reproduction.

Soybean variety OT21325816 in one embodiment of the present inventioncarries one or more transgenes, for example, a glyphosate tolerancetransgene, an auxin herbicide (e.g., 2,4-D, dicamba, etc.) tolerancegene, a glufosinate tolerance gene, insect resistance transgene(s), orother transgenes. In another embodiment of the invention, the soybeandoes not carry any herbicide tolerance traits. In yet another embodimentof the invention, the soybean does not carry any transgenes, but carriesalleles for disease and/or pest resistance, such as aphid resistance,cyst nematode resistance or the like. In still another embodiment, thesoybean carries both alleles and transgenes providing desired traits.

Soybean Variety OT21325816 Breeding and Production Methods

The present invention provides methods for producing soybean seed, orplants grown therefrom, by crossing the soybean variety OT21325816 withitself or a second variety. These methods can be used for propagation ofthe soybean variety OT21325816, or can be used to produceOT21325816-derived hybrid soybean seeds and the plants grown therefrom.Hybrid soybean plants can be used in the commercial production of soyproducts or may be advanced in certain breeding protocols for theproduction of additional novel soybean varieties by crossing the soybeanvariety OT21325816-derived soybean plant with itself or another soybeanplant to yield an additional soybean variety OT21325816-derived soybeanprogeny seed. This crossing process can be repeated one or more times togenerate additional soybean varieties. A hybrid plant can also be usedas a recurrent parent at any given stage in a backcrossing protocolduring the production of the soybean variety OT21325816 which comprisesan added desired trait.

In some embodiments, the present invention provides for using theOT21325816 soybean plant, or part thereof, or a soybean plant having thephysiological and morphological characteristics of the OT21325816soybean plant, as a source of breeding material for developing anOT21325816-derived soybean plant in a soybean breeding program usingplant breeding techniques. A soybean plant having the physiological andmorphological characteristics of soybean variety OT21325816 may includea plant having the characteristics set forth in Table 2 when grown inthe same environmental conditions. Plant breeding techniques useful inthe developing soybean plants include, but are not limited to, singleseed descent, modified single seed descent, recurrent selection,reselection, mass selection, bulk selection, backcrossing, pedigreebreeding, mutation breeding, restriction fragment length polymorphismenhanced selection, genetic marker enhanced selection, making doublehaploids and transformation. Transformation includes introduction ofnon-homologous DNA into a plant genome and targeted manipulation of theplant genome using gene editing techniques such as described herein.Plant breeding techniques are known to the art and have been describedin the literature. For example, see U.S. Pat. Nos. 6,143,954; 7,803,996;and 7,807,884, which, along with the references cited therein, areincorporated by reference herein.

Selection of soybean plants for breeding is not necessarily dependent onthe phenotype of a plant and instead can be based on geneticinvestigations. For example, one may utilize a suitable genetic markerwhich is closely genetically linked to a trait of interest. One of thesemarkers may therefore be used to identify the presence or absence of atrait in the offspring of a particular cross, and hence may be used inselection of progeny for continued breeding. This technique may commonlybe referred to as “marker assisted selection.” Any other type of geneticmarker or other assay which is able to identify the relative presence orabsence of a trait of interest in a plant may also be useful forbreeding purposes. Procedures for marker assisted selection applicableto the breeding of soybeans are well known in the art. Such methods willbe of particular utility in the case of recessive traits and variablephenotypes, or where conventional assays may be more expensive, timeconsuming or otherwise disadvantageous. Types of genetic markers whichcould be used in accordance with the invention include, but are notnecessarily limited to, Simple Sequence Length Polymorphisms (SSLPs),Restriction Fragment Length Polymorphisms (RFLPs), Randomly AmplifiedPolymorphic DNAs (RAPDs), DNA Amplification Fingerprinting (DAF),Sequence Characterized Amplified Regions (SCARs), Arbitrary PrimedPolymerase Chain Reaction (AP-PCR), Amplified Fragment LengthPolymorphisms (AFLPs) (see, e.g., EP534858, specifically incorporatedherein by reference in its entirety), Simple Sequence Repeats (SSRs,also referred to as “Microsatellites”), and Single NucleotidePolymorphisms (SNPs).

Many qualitative characteristics also have potential use asphenotype-based genetic markers in soybeans; however, some or many maynot differ among varieties commonly used as parents. The most widelyused genetic markers are flower color (purple dominant to white),pubescence color (brown dominant to gray), and pod color (brown dominantto tan). The association of purple hypocotyl color with purple flowersand green hypocotyl color with white flowers is commonly used toidentify hybrids in the seedling stage. Differences in maturity, height,hilum color, pubescence color, pod wall color, and pest resistancebetween parents can also be used to verify hybrid plants.

Soybean variety OT21325816 represents a novel base genetic variety intowhich a new desired locus or trait may be introduced by introgression.Backcrossing and direct transformation represent two important methodsthat can be used to accomplish such an introgression. In certainembodiments of the present invention, plants of soybean varietyOT21325816 are provided modified to include one or more desiredheritable traits.

Plants of the subject invention including one or more desired heritabletraits may, in one embodiment, be developed by a plant breedingtechnique called backcrossing, wherein essentially all of the desiredphysiological and morphological characteristics of a variety arerecovered, when grown in the same environmental conditions, in additionto a genetic locus comprising the desired trait transferred into theplant via the backcrossing technique. Backcrossing methods can be usedwith the present invention to improve or introduce a desired trait intosoybean variety OT21325816. The parental soybean plant which contributesthe locus for the desired characteristic is termed the nonrecurrent ordonor parent. This terminology refers to the fact that the nonrecurrentparent is used one time in the backcross protocol and therefore does notrecur. The parental soybean plant (e.g., soybean variety OT21325816) towhich the locus or loci from the nonrecurrent parent are transferred isknown as the recurrent parent as it is used for several rounds in thebackcrossing protocol (see, e.g., Poehlman (eds), “Backcross Breeding,”In: Breeding Field Crops, 4th Ed., pp. 172-175, 1995; Fehr, In:Principles of Variety Development, Theory and Technique (Vol 1) and CropSpecies Soybean (Vol 2), Iowa State Univ., Macmillian Pub. Co., NY,360-376, 1987; Fehr, In: Soybeans: Improvement, Production and Uses, 2dEd., Monograph 16:249, 1987).

In a typical backcross protocol, the original variety of interest(recurrent parent, e.g., soybean variety OT21325816) is crossed to asecond variety (nonrecurrent parent) that carries the single locus ofinterest to be transferred to produce F1 progeny plants. The resultingF1 progeny from this cross are then selected that have the desired traitand crossed again to the recurrent parent to produce backcross progenyplants having the desired trait and physiological and morphologicalcharacteristics of the recurrent parent. The process is repeated until asoybean plant is obtained wherein essentially all of the desiredmorphological and physiological characteristics of the recurrent parentare recovered in the converted plant, in addition to the transferredlocus comprising the desired trait from the nonrecurrent parent.

The selection of a suitable recurrent parent is an important step for asuccessful backcrossing procedure. The goal of a backcross protocol isto alter or substitute a single or a very limited number of traits orcharacteristics into the original variety. To accomplish this, a singlelocus of the recurrent variety is modified or substituted with thedesired locus from the nonrecurrent parent, while retaining essentiallyall of the rest of the desired genetic, and therefore the desiredphysiological and morphological constitution of the original variety.The choice of the particular nonrecurrent parent will depend on thepurpose of the backcross; one of the major purposes is to add somecommercially desirable, agronomically important trait to the plant. Theexact backcrossing protocol will depend on the characteristic or traitbeing altered to determine an appropriate testing protocol. Althoughbackcrossing methods are simplified when the characteristic beingtransferred is a dominant allele, a recessive allele may also betransferred. In this instance it may be necessary to introduce a test ofthe progeny to determine if the desired characteristic has beensuccessfully transferred.

Soybean varieties can also be developed from more than two parents (see,e.g., Fehr, In: Soybeans: Improvement, Production and Uses, 2d Ed.,Manograph 16:249, 1987). The technique, known as modified backcrossing,uses different recurrent parents during the backcrossing. Modifiedbackcrossing can be used to replace the original recurrent parent with avariety having certain more desirable characteristics or multipleparents may be used to obtain different desirable characteristics fromeach.

Many single locus traits have been identified that are not regularlyselected for in the development of a new inbred but that can be improvedby backcrossing techniques. Single locus traits may or may not betransgenic; examples of these traits include, but are not limited to,male sterility, herbicide tolerance, insect resistance, modified fattyacid metabolism, modified carbohydrate metabolism and resistance tobacterial disease, fungal disease or viral disease.

Direct selection may be applied where the single locus acts as adominant trait. An example of a dominant trait is an herbicide tolerancetrait. For this selection process, the progeny of the initial cross aresprayed with the herbicide prior to the backcrossing. The sprayingeliminates any plants which do not have the desired herbicide tolerancecharacteristic, and only those plants which have the herbicide tolerancegene are used in the subsequent backcross. This process is then repeatedfor all additional backcross generations.

In other embodiments of the present invention, plants of the subjectinvention including one or more desired heritable traits may bedeveloped by direct transformation, genetic editing or modification ofsoybean variety OT21325816, or through the use of backcrossingapproaches as described herein, for example, to introgress a transgenictrait into soybean variety OT21325816. Accordingly, in one embodiment ofthe present invention a method of producing a plant of soybean varietyOT21325816 comprising an added desired trait is provided, where themethod comprises introducing at least one transgene or geneticmodification conferring the desired trait into variety OT21325816.Plants, seeds and plant parts produced by the methods described hereinare also provided.

In general, methods to transform, modify, edit or alter plant endogenousgenomic DNA include altering the plant native DNA sequence or apre-existing transgenic sequence including regulatory elements, codingand non-coding sequences. These methods can be used, for example, totarget nucleic acids to pre-engineered target recognition sequences inthe genome. Such pre-engineered target sequences may be introduced bygenome editing or modification. As an example, a genetically modifiedplant variety is generated using “custom” or engineered endonucleasessuch as meganucleases produced to modify plant genomes (see e.g., WO2009/114321; Gao et al. (2010) Plant Journal 1:176-187). Anothersite-directed engineering method is through the use of zinc fingerdomain recognition coupled with the restriction properties ofrestriction enzyme. See e.g., Urnov, et al., (2010) Nat Rev Genet.11(9):636-46; Shukla, et al., (2009) Nature 459 (7245):437-41. Atranscription activator-like (TAL) effector-DNA modifying enzyme (TALEor TALEN) is also used to engineer changes in plant genome. See e.g.,US20110145940, Cermak et al., (2011) Nucleic Acids Res. 39(12) and Bochet al., (2009), Science 326(5959): 1509-12. Site-specific modificationof plant genomes can also be performed using the bacterial type IICRISPR (clustered regularly interspaced short palindromic repeats)/Cas(CRISPR-associated) system. See e.g., Belhaj et al., (2013), PlantMethods 9: 39; The Cas9/guide RNA-based system allows targeted cleavageof genomic DNA guided by a customizable small noncoding RNA in plants(see e.g., WO 2015026883A1, incorporated herein by reference).

Plant transformation methods may involve the construction of anexpression vector. Such a vector or recombinant construct comprises aDNA sequence that contains a coding sequence, such as a protein and/orRNA coding sequence under the control of or operatively linked to aregulatory element, for example a promoter. The vector or construct maycontain one or more coding sequences and one or more regulatoryelements.

In some embodiments, introducing at least one transgene conferring thedesired trait comprises transforming a soybean plant, or part thereof,of variety OT21325816 with one or more transgenes that confer at leastone desired trait. In another embodiment, introducing at least onetransgene conferring the desired trait comprises use of backcrossing tointrogress a transgenic trait into soybean variety OT21325816. Anotherembodiment is the product produced by this process, wherein the productcomprises the at least one desired trait and all of the physiologicaland morphological characteristics of soybean variety OT21325816 whengrown in the same location and in the same environmental conditions.

In one embodiment the desired trait may be one or more of malesterility, site-specific recombination, abiotic stress tolerance,herbicide tolerance, insect or pest resistance, disease resistance,fungal resistance, modified fatty acid metabolism, and modifiedcarbohydrate metabolism. The specific gene may be any known in the artor listed herein, including: a polynucleotide conferring tolerance toglyphosate, phenoxyacetate auxins, pyridyloxyacetate auxins,phenoxyproprionate auxins, phenoxybutanoate auxins, sulfonylurea,imidazalinone, dicamba, glufosinate, cyclohexone, triazine, andbenzonitrile; a polynucleotide encoding a Bacillus thuringiensispolypeptide (e.g. Cry1F and/or Cry1Ac), a polynucleotide encodingphytase, FAD-2, FAD-3, galactinol synthase or a raffinose syntheticenzyme; or a polynucleotide conferring resistance to soybean cystnematode, brown stem rot, phytophthora root rot, soybean mosaic virus orsudden death syndrome.

Various genetic elements can be introduced into the plant genome usingtransformation. These elements include, but are not limited to: genes;coding sequences; inducible, constitutive, and tissue specificpromoters; enhancing sequences; and signal and targeting sequences. Forexample, see the traits, genes and transformation methods listed in U.S.Pat. No. 6,118,055, incorporated herein by reference. The most prevalenttypes of plant transformation involve the construction of an expressionvector. Such a vector comprises a DNA sequence that contains a geneunder the control of or operatively linked to a regulatory element, forexample a promoter. The vector may contain one or more genes and one ormore regulatory elements.

Plant transformation techniques which result in the generation oftransgenic plants are known in the art. These techniques include, butare not limited to:

(1) Projectile bombardment or microprojectile-mediated delivery. Thisprocedure involves propelling inert or biologically active particlescomplexed with DNA at plant cells, wherein the particles penetrate theouter surface of the cell and the DNA is incorporated within the genomeof the plant cell. See e.g., Klein et al., (1987) Nature 327: 70-73;Tomes et al., Plant Cell, Tissue & Organ Culture: Fundamental Methods,eds. Gambourg and Phillips (1995) (Springer-Velag, Berlin); Gordon-Kimet al., (1990) Plant Cell 2:603-618; U.S. Pat. Nos. 4,945,050;5,879,918; 5,932,782; 5,015,580; 5,550,318; 5,538,880; 6,160,208;6,399,861; and 6,403,865;

(2) Microinjection of plant cell protoplasts or embryogenic callus,including the use of silicon carbide mediated DNA uptake. See e.g.,Crossway et al., (1985) Molecular General Genetics 202:179; Kaeppler etal. (1990) Plant Cell Reporter 9:415-418;

(3) Direct gene transfer. See e.g., International Patent Application No.WO85/01856 and European Patent Application No. 0 275 069;

(4) Electroporation, calcium mediated, or PEG precipitation ofprotoplasts or cells comprising partial cell walls. See e.g., Fromm etal., (1985) Proceedings of the National Academy of Sciences 82: 5824;Paszkowski et al., (1984) European Molecular Biological Organization 3:2717-2722; Potrykus et al. (1985) Molecular General Genetics199:169-177; Shimamoto (1989) Nature 338:274-276; D'Halluin et al.(1992) Plant Cell 4: 1 495-1505; and U.S. Pat. No. 5,384,253.

(5) Aerosol beam technology, which employs the jet expansion of an inertgas as it passes from a region of higher gas pressure to a region oflower gas pressure through a small orifice. The expanding gasaccelerates aerosol droplets containing DNA molecules at supersonicspeeds into a cell or tissue. See e.g., Held et al., U.S. Pat. Nos.6,809,232; 7,067,716; and 7,026,286.

(6) Agrobacterium-mediated transformations of plants are also included.Agrobacterium-mediated transformation is described in, for example,Horsch et al., (1984) Science 233:496-498, and Fraley et al., (1983)Proc. Nat. Acad. Sci. USA 80:4803 and U.S. Pat. Nos. 5,824,877;5,981,840, and 6,384,301; Ishida et al., (1996) Nature Biotechnology14:745-750. Generally, the Agrobacterium transformation system is usedto engineer dicotyledonous plants see Bevan et al (1982) Ann. Rev. Genet16:357-384; Rogers et al., (1986) Methods Enzymol. 118:627-641). TheAgrobacterium transformation system may also be used to transform, aswell as transfer, DNA to monocotyledonous plants and plant cells. SeeU.S. Pat. No. 5,591,616; Hemalsteen et al., (1984) EMBO J 3:3039-3041;Hooykass-Van Slogteren et al., (1984) Nature 3 11: 763-764; Grimsley etal., (1987) Nature 325: 1677-179; Boulton et al., (1989) Plant Mol.Biol. 12:3 1-40.; and Gould et al., (1991) Plant Physiol. 95:426-434. Inaddition, gene transfer may be achieved using non-Agrobacterium bacteriaor viruses such as Rhizobium sp. NGR234, Sinorhizobium meliloti,Mesorhizobium loti, potato virus X, cauliflower mosaic virus and cassavavein mosaic virus and/or tobacco mosaic virus, See, e.g., Chung et al.,(2006) Trends Plant Sci. 1 1(1): 1-4; U.S. Pat. Nos. 6,660,500;6,462,255; 5,889,190 and 5,889,101.

Soybean transformation is described in particular in a number ofpublications. An example of an exemplary soybean transformationtechnique includes the use of Agrobacterium-mediated planttransformation. One example of soybean transformation comprisesinfecting half-seed explants of soybean with Agrobacterium tumefacienscontaining a transgene and regenerating the half-seed explants in vitroon selection medium. See U.S. Pat. No. 7,473,822 and Paz et al., (2006)Plant Cell Reports 25: 206-213. A second example ofAgrobacterium-mediated soybean transformation employs the use ofglufosinate as the selection system, thereby resulting in an enhancedtransformation efficiency. See Zeng et al., (2004) Plant Cell Rep22:478-482.

After effecting delivery of exogenous DNA to recipient cells, the nextsteps generally concern identifying the transformed cells for furtherculturing and plant regeneration. In order to improve the ability toidentify transformants, one may desire to employ a selectable orscreenable marker gene with the transformation vector used to generatethe transformant. In this case, the potentially transformed cellpopulation can be assayed by exposing the cells to a selective agent oragents, or the cells can be screened for the desired marker gene trait.

Cells that survive the exposure to the selective agent, or cells thathave been scored positive in a screening assay, may be cultured in mediathat supports regeneration of plants. In some embodiments, any suitableplant tissue culture media (e.g., MS and N6 media) may be modified byincluding further substances, such as growth regulators. Tissue may bemaintained on a basic media with growth regulators until sufficienttissue is available to begin plant regeneration efforts, or followingrepeated rounds of manual selection, until the morphology of the tissueis suitable for regeneration (e.g., at least 2 weeks), then transferredto media conducive to shoot formation. Cultures are transferredperiodically until sufficient shoot formation has occurred. Once shootsare formed, they are transferred to media conducive to root formation.Once sufficient roots are formed, plants can be transferred to soil forfurther growth and maturity.

To confirm the presence of a transgene in the regenerating plants, avariety of assays may be performed. Such assays include, for example:molecular biological assays, such as Southern and Northern blotting andPCR; biochemical assays, such as detecting the presence of a proteinproduct, e.g., by immunological means (e.g., ELISA and/or Western blots)or by enzymatic function; plant part assays, such as leaf or rootassays; and analysis of the phenotype of the whole regenerated plant.

Through the transformation of soybean, the expression of genes can bealtered to enhance disease resistance, insect resistance, herbicidetolerance, agronomic, grain quality and other desired traits.Transformation can also be used to insert DNA sequences which control orhelp control male-sterility. DNA sequences native to soybean as well asnon-native DNA sequences can be transformed into soybean and used toalter levels of native or non-native proteins. Various promoters,targeting sequences, enhancing sequences, and other DNA sequences can beinserted into the genome for the purpose of altering the expression ofproteins. Reduction of the activity of specific genes (also known asgene silencing, or gene suppression) is desirable for several aspects ofgenetic engineering in plants.

Many techniques for gene silencing are well known to one of skill in theart, including but not limited to knock-outs (such as by insertion of atransposable element such as mu (Vicki Chandler, The Maize Handbook ch.118 (Springer-Verlag 1994)) or other genetic elements such as a FRT, Loxor other site specific integration site, antisense technology (see,e.g., Sheehy et al. (1988) PNAS USA 85:8805-8809; and U.S. Pat. Nos.5,107,065; 5,453,566; and 5,759,829); co-suppression (e.g., Taylor(1997) Plant Cell 9:1245; Jorgensen (1990) Trends Biotech.8(12):340-344; Flavell (1994) PNAS USA 91:3490-3496; Finnegan et al.(1994) Bio/Technology 12: 883-888; and Neuhuber et al. (1994) Mol. Gen.Genet. 244:230-241); RNA interference (Napoli et al. (1990) Plant Cell2:279-289; U.S. Pat. No. 5,034,323; Sharp (1999) Genes Dev. 13:139-141;Zamore et al. (2000) Cell 101:25-33; and Montgomery et al. (1998) PNASUSA 95:15502-15507), virus-induced gene silencing (Burton, et al. (2000)Plant Cell 12:691-705; and Baulcombe (1999) Curr. Op. Plant Bio.2:109-113); target-RNA-specific ribozymes (Haseloff et al. (1988) Nature334: 585-591); hairpin structures (Smith et al. (2000) Nature407:319-320; WO 99/53050; and WO 98/53083); MicroRNA (Aukerman & Sakai(2003) Plant Cell 15:2730-2741); ribozymes (Steinecke et al. (1992) EMBOJ. 11:1525; and Perriman et al. (1993) Antisense Res. Dev. 3:253);oligonucleotide-mediated targeted modification (e.g., WO 03/076574 andWO 99/25853); Zn-finger targeted molecules (e.g., WO 01/52620; WO03/048345; and WO 00/42219); and other methods or combinations of theabove methods known to those of skill in the art.

Exemplary nucleotide sequences or encoded polypeptides that may bealtered or introduced by genetic engineering to provide desired traitsinclude, but are not limited to, those categorized below.

1. Genes or Encoded Proteins that Confer Resistance to Pests or Disease.

(A) Plant Disease Resistance Genes. Plant defenses are often activatedby specific interaction between the product of a disease resistance gene(R) in the plant and the product of a corresponding avirulence (Avr)gene in the pathogen. A plant variety can be transformed with clonedresistance gene to engineer plants that are resistant to specificpathogen strains. Examples of such genes include, the tomato Cf-9 genefor resistance to Cladosporium flavum (Jones et al., 1994 Science266:789), tomato Pto gene, which encodes a protein kinase, forresistance to Pseudomonas syringae pv. tomato (Martin et al., 1993Science 262:1432), and Arabidopsis RSSP2 gene for resistance toPseudomonas syringae (Mindrinos et al., 1994 Cell 78:1089).

(B) A Bacillus thuringiensis protein, a derivative thereof or asynthetic polypeptide modeled thereon, such as, a nucleotide sequence ofa Bt δ-endotoxin gene (Geiser et al., 1986 Gene 48:109). Moreover, DNAmolecules encoding δ-endotoxin genes can be purchased from American TypeCulture Collection (Rockville, Md.), under ATCC accession numbers, e.g.,40098, 67136, 31995 and 31998. Other non-limiting examples of Bacillusthuringiensis transgenes being genetically engineered are given in thefollowing patents, patent applications and publications and hereby areincorporated by reference for this purpose: U.S. Pat. Nos. 5,188,960;5,689,052; 5,880,275; 5,986,177; 7,105,332; 7,208,474; WO 91/14778; WO99/31248; WO 01/12731; WO 99/24581; WO 97/40162 and U.S. applicationSer. Nos. 10/032,717; 10/414,637; 11/018,615; 11/404,297; 11/404,638;11/471,878; 11/780,501; 11/780,511; 11/780,503; 11/953,648; 11/953,648;and Ser. No. 11/957,893, and Estruch, et al., 1996. Proc. Natl. Acad.Sci. 93:5389.

(C) A lectin, such as, nucleotide sequences of several Clivia miniatamannose-binding lectin genes (Van Damme et al., 1994 Plant Molec. Biol.24:825).

(D) A vitamin binding protein, such as avidin and avidin homologs whichare useful as larvicides against insect pests. See U.S. Pat. No.5,659,026.

(E) An enzyme inhibitor, e.g., a protease inhibitor or an amylaseinhibitor. Examples of such genes include, a rice cysteine proteinaseinhibitor (Abe et al., 1987 J. Biol. Chem. 262:16793), a tobaccoproteinase inhibitor I (Huub et al., 1993 Plant Molec. Biol. 21:985),and an α-amylase inhibitor Sumitani et al., 1993 Biosci. Biotech.Biochem. 57:1243).

(F) An insect-specific hormone or pheromone such as an ecdysteroid andjuvenile hormone a variant thereof, a mimetic based thereon, or anantagonist or agonist thereof, such as, baculovirus expression of clonedjuvenile hormone esterase, an inactivator of juvenile hormone (Hammocket al., 1990 Nature 344:458).

(G) An insect-specific peptide or neuropeptide which, upon expression,disrupts the physiology of the affected pest. Examples of such genesinclude, an insect diuretic hormone receptor (Regan, 1994), anallostatin identified in Diploptera punctata (Pratt, 1989),insect-specific, paralytic neurotoxins (U.S. Pat. No. 5,266,361).

(H) An insect-specific venom produced in nature by a snake, a wasp,etc., such as, a scorpion insectotoxic peptide (Pang, 1992 Gene116:165).

(I) An enzyme responsible for a hyperaccumulation of monoterpene, asesquiterpene, a steroid, hydroxamic acid, a phenylpropanoid derivativeor another non-protein molecule with insecticidal activity.

(J) An enzyme involved in the modification, including thepost-translational modification, of a biologically active molecule; forexample, glycolytic enzyme, a proteolytic enzyme, a lipolytic enzyme, anuclease, a cyclase, a transaminase, an esterase, a hydrolase, aphosphatase, a kinase, a phosphorylase, a polymerase, an elastase, achitinase and a glucanase, whether natural or synthetic. Examples ofsuch genes include, a callas gene (PCT published applicationWO93/02197), chitinase-encoding sequences (which can be obtained, forexample, from the ATCC under accession numbers 3999637 and 67152),tobacco hookworm chitinase (Kramer et al., 1993 Insect Molec. Biol.23:691) and parsley ubi4-2 polyubiquitin gene (Kawalleck et al., 1993Plant Molec. Biol. 21:673).

(K) A molecule that stimulates signal transduction. Examples of suchmolecules include, nucleotide sequences for mung bean calmodulin cDNAclones (Botella et al., 1994 Plant Molec. Biol. 24:757) and a nucleotidesequence of a maize calmodulin cDNA clone (Griess et al., 1994 PlantPhysiol. 104:1467).

(L) A hydrophobic moment peptide. See U.S. Pat. Nos. 5,659,026 and5,607,914, the latter teaches synthetic antimicrobial peptides thatconfer disease resistance.

(M) A membrane permease, a channel former or a channel blocker, such as,a cecropin-β lytic peptide analog (Jaynes et al., 1993 Plant Sci. 89:43)which renders transgenic tobacco plants resistant to Pseudomonassolanacearum.

(N) A viral-invasive protein or a complex toxin derived there from. Forexample, the accumulation of viral coat proteins in transformed plantcells imparts resistance to viral infection and/or disease developmenteffected by the virus from which the coat protein gene is derived, aswell as by related viruses. Coat protein-mediated resistance has beenconferred upon transformed plants against alfalfa mosaic virus, cucumbermosaic virus, tobacco streak virus, potato virus X, potato virus Y,tobacco etch virus, tobacco rattle virus and tobacco mosaic virus. See,for example, Beachy et al. (1990) Ann. Rev. Phytopathol. 28:451.

(O) An insect-specific antibody or an immunotoxin derived therefrom.Thus, an antibody targeted to a critical metabolic function in theinsect gut would inactivate an affected enzyme, killing the insect. Forexample, Taylor et al. (1994) Abstract #497, Seventh Int'l. Symposium onMolecular Plant-Microbe Interactions, shows enzymatic inactivation intransgenic tobacco via production of single-chain antibody fragments.

(P) A virus-specific antibody. See, for example, Tavladoraki et al.(1993) Nature 266:469, which shows that transgenic plants expressingrecombinant antibody genes are protected from virus attack.

(Q) A developmental-arrestive protein produced in nature by a pathogenor a parasite. Thus, fungal endo α-1,4-D polygalacturonases facilitatefungal colonization and plant nutrient release by solubilizing plantcell wall homo-α-1,4-D-galacturonase (Lamb et al., 1992) Bio/Technology10:1436. The cloning and characterization of a gene which encodes a beanendopolygalacturonase-inhibiting protein is described by Toubart et al.(1992 Plant J. 2:367).

(R) A developmental-arrestive protein produced in nature by a plant,such as, the barley ribosome-inactivating gene has an increasedresistance to fungal disease (Longemann et al., 1992). Bio/Technology10:3305.

(S) A small RNA (e.g., antisense, hairpin, siRNA, or miRNA) thatinhibits expression of a pathogen gene necessary for the pathogen tosurvive or thrive.

2. Genes that Confer Tolerance to a Herbicide.

(A) Genes encoding resistance or tolerance to a herbicide that inhibitsthe growing point or meristem, such as an imidazalinone or asulfonylurea. Exemplary genes in this category code for mutant ALS (Leeet al., 1988 EMBOJ. 7:1241) and AHAS enzyme (Miki et al., 1990 Theor.Appl. Genet. 80:449).

(B) One or more additional genes encoding resistance or tolerance toglyphosate imparted by mutant EPSP synthase and aroA genes, or throughmetabolic inactivation by genes such as GAT (glyphosateacetyltransferases or GOX (glyphosate oxidase) and other phosphonocompounds such as glufosinate (PAT and bar genes), and pyridinoxy orphenoxy proprionic acids and cyclohexones (ACCase inhibitor encodinggenes). See, for example, U.S. Pat. No. 4,940,835, which discloses thenucleotide sequence of a form of EPSP which can confer glyphosatetolerance. A DNA molecule encoding a mutant aroA gene can be obtainedunder ATCC accession number 39256, and the nucleotide sequence of themutant gene is disclosed in U.S. Pat. No. 4,769,061. European patentapplication No. 0 333 033 and U.S. Pat. No. 4,975,374 disclosenucleotide sequences of glutamine synthetase genes which confertolerance to herbicides such as L-phosphinothricin. The nucleotidesequence of a phosphinothricinacetyl-transferase gene is provided inEuropean application No. 0 242 246. De Greef et al. (1989)Bio/Technology 7:61 describes the production of transgenic plants thatexpress chimeric bar genes coding for phosphinothricin acetyltransferase activity. Exemplary genes conferring tolerance to phenoxyproprionic acids and cyclohexones, such as sethoxydim and haloxyfop, arethe Accl-S1, Accl-S2 and Accl-S3 genes described by Marshall et al.(1992) Theor. Appl. Genet. 83:435.

(C) Genes encoding resistance or tolerance to a herbicide that inhibitsphotosynthesis, such as a triazine (psbA and gs+ genes) and abenzonitrile (nitrilase gene). Przibilla et al. (1991) Plant Cell 3:169describes the use of plasmids encoding mutant psbA genes to transformChlamydomonas. Nucleotide sequences for nitrilase genes are disclosed inU.S. Pat. No. 4,810,648, and DNA molecules containing these genes areavailable under ATCC accession numbers 53435, 67441 and 67442. Cloningand expression of DNA coding for a glutathione 5-transferase isdescribed by Hayes et al. (1992) Biochem. J. 285:173.

(D) Genes encoding resistance or tolerance to a herbicide that bind tohydroxyphenylpyruvate dioxygenases (HPPD), enzymes which catalyze thereaction in which para-hydroxyphenylpyruvate (HPP) is transformed intohomogentisate. This includes herbicides such as isoxazoles (EP418175,EP470856, EP487352, EP527036, EP560482, EP682659, U.S. Pat. No.5,424,276), in particular isoxaflutole, which is a selective herbicidefor maize, diketonitriles (EP496630, EP496631), in particular2-cyano-3-cyclopropyl-1-(2-SO2CH3-4-CF3 phenyl)propane-1,3-dione and2-cyano-3-cyclopropyl-1-(2-SO2CH3-4-2,3Cl2phenyl)propane-1,3-dione,triketones (EP625505, EP625508, U.S. Pat. No. 5,506,195), in particularsulcotrione, or else pyrazolinates. A gene that produces anoverabundance of HPPD in plants can provide tolerance or resistance tosuch herbicides, including, for example, genes described at U.S. Pat.Nos. 6,268,549 and 6,245,968 and U.S. publication No. 20030066102.

(E) Genes encoding resistance or tolerance to phenoxyacetate auxinherbicides, such as 2, 4-dichlorophenoxyacetic acid (2,4-D) and whichmay also confer resistance or tolerance to “fop” herbicides. Examples ofsuch genes include the α-ketoglutarate-dependent dioxygenase enzyme(AAD-1) gene, described at U.S. Pat. No. 7,838,733.

(F) Genes encoding resistance or tolerance to phenoxyacetate auxinherbicides, such as 2, 4-dichlorophenoxyacetic acid (2,4-D) and whichmay also confer resistance or tolerance to pyridyloxyacetate auxins(such as fluroxypyr and triclopyr), phenoxyproprionate auxins (such asMCPA and dichloprop), phenoxybutanoate auxins (such as 2,4-DB). Examplesof such genes include the α-ketoglutarate-dependent dioxygenase enzyme(AAD-12) gene, described in U.S. Pat. No. 8,283,522.

(G) Genes encoding resistance or tolerance to dicamba, such as dicambamonooxygenase (DMO) from Pseudomonas maltophilia which is involved inthe conversion of a herbicidal form of the herbicide dicamba to anon-toxic 3,6-dichlorosalicylic acid and thus may be used for producingplants tolerant to this herbicide. See, e.g., U.S. Patent ApplicationNo: 20030135879.

3. Genes that Confer or Contribute to a Value-Added Trait.

(A) Modified fatty acid metabolism, for example, by transforming maizeor Brassica with a small RNA or stearoyl-ACP desaturase to increasestearic acid content of the plant (Knultzon et al., 1992) Proc. Nat.Acad. Sci. USA 89:2624.

(B) Decreased phytate content

(1) Introduction of a phytase-encoding gene would enhance breakdown ofphytate, adding more free phosphate to the transformed plant, such asthe Aspergillus niger phytase gene (Van Hartingsveldt et al., 1993 Gene127:87).

(2) A gene could be introduced that reduces phytate content. In maize,this, for example, could be accomplished by cloning and thenreintroducing DNA associated with the single allele which is responsiblefor maize mutants characterized by low levels of phytic acid (Raboy etal., 1990 Maydica 35:383).

(C) Modified carbohydrate composition effected, for example, bytransforming plants with a gene coding for an enzyme that alters thebranching pattern of starch. Examples of such enzymes include,Streptococcus mucus fructosyltransferase gene (Shiroza et al., 1988) J.Bacteriol. 170:810, Bacillus subtilis levansucrase gene (Steinmetz etal., 1985 Mol. Gen. Genel. 200:220), Bacillus licheniformis α-amylase(Pen et al., 1992 Bio/Technology 10:292), tomato invertase genes (Elliotet al., 1993), barley amylase gene (Sogaard et al., 1993 J. Biol. Chem.268:22480), and maize endosperm starch branching enzyme II (Fisher etal., 1993 Plant Physiol. 102:1045).

(D) Altered antioxidant content or composition, such as alteration oftocopherol or tocotrienols. For example, see U.S. Pat. No. 6,787,683,US2004/0034886 and WO 00/68393 involving the manipulation of antioxidantlevels, and WO 03/082899 through alteration of a homogentisate geranylgeranyl transferase (HGGT).

(E) Altered essential seed amino acids. For example, see U.S. Pat. No.6,127,600 (method of increasing accumulation of essential amino acids inseeds), U.S. Pat. No. 6,080,913 (binary methods of increasingaccumulation of essential amino acids in seeds), U.S. Pat. No. 5,990,389(high lysine), WO 99/40209 (alteration of amino acid compositions inseeds), WO 99/29882 (methods for altering amino acid content ofproteins), U.S. Pat. No. 5,850,016 (alteration of amino acidcompositions in seeds), WO 98/20133 (proteins with enhanced levels ofessential amino acids), U.S. Pat. No. 5,885,802 (high methionine), U.S.Pat. No. 5,885,801 (high threonine), U.S. Pat. No. 6,664,445 (plantamino acid biosynthetic enzymes), U.S. Pat. No. 6,459,019 (increasedlysine and threonine), U.S. Pat. No. 6,441,274 (plant tryptophansynthase beta subunit), U.S. Pat. No. 6,346,403 (methionine metabolicenzymes), U.S. Pat. No. 5,939,599 (high sulfur), U.S. Pat. No. 5,912,414(increased methionine), WO 98/56935 (plant amino acid biosyntheticenzymes), WO 98/45458 (engineered seed protein having higher percentageof essential amino acids), WO 98/42831 (increased lysine), U.S. Pat. No.5,633,436 (increasing sulfur amino acid content), U.S. Pat. No.5,559,223 (synthetic storage proteins with defined structure containingprogrammable levels of essential amino acids for improvement of thenutritional value of plants), WO 96/01905 (increased threonine), WO95/15392 (increased lysine), US2003/0163838, US2003/0150014,US2004/0068767, U.S. Pat. No. 6,803,498, WO 01/79516.

4. Genes that Control Male-Sterility.

There are several methods of conferring genetic male sterilityavailable, such as multiple mutant genes at separate locations withinthe genome that confer male sterility, as disclosed in U.S. Pat. Nos.4,654,465 and 4,727,219 to Brar et al. and chromosomal translocations asdescribed by Patterson in U.S. Pat. Nos. 3,861,709 and 3,710,511. Inaddition to these methods, Albertsen et al., U.S. Pat. No. 5,432,068,describe a system of nuclear male sterility which includes: identifyinga gene which is critical to male fertility; silencing this native genewhich is critical to male fertility; removing the native promoter fromthe essential male fertility gene and replacing it with an induciblepromoter; inserting this genetically engineered gene back into theplant; and thus creating a plant that is male sterile because theinducible promoter is not “on” resulting in the male fertility gene notbeing transcribed. Fertility is restored by inducing, or turning “on”,the promoter, which in turn allows the gene that confers male fertilityto be transcribed.

(A) Introduction of a deacetylase gene under the control of atapetum-specific promoter and with the application of the chemicalN—Ac-PPT (WO 01/29237).

(B) Introduction of various stamen-specific promoters (WO 92/13956, WO92/13957).

(C) Introduction of the barnase and the barstar gene (Paul et al. PlantMol. Biol. 19:611-622, 1992).

For additional examples of nuclear male and female sterility systems andgenes, see also, U.S. Pat. Nos. 5,859,341; 6,297,426; 5,478,369;5,824,524; 5,850,014; and 6,265,640; all of which are herebyincorporated by reference.

5. Genes that affect abiotic stress resistance (including but notlimited to enhancement of nitrogen utilization efficiency, alterednitrogen responsiveness, drought resistance or tolerance, coldresistance or tolerance, and salt resistance or tolerance) and increasedyield under stress. For example, see: WO 00/73475 where water useefficiency is altered through alteration of malate; U.S. Pat. Nos.5,892,009, 5,965,705, 5,929,305, 5,891,859, 6,417,428, 6,664,446,6,706,866, 6,717,034, 6,801,104, WO 00/060089, WO 01/026459, WO01/035725, WO 01/034726, WO 01/035727, WO 01/036444, WO 01/036597, WO01/036598, WO 02/015675, WO 02/017430, WO 02/079403, WO 03/013227, WO03/013228, WO 03/014327, WO 04/031349, WO 04/076638, WO 98/09521, and WO99/38977 describing genes, including CBF genes and transcription factorseffective in mitigating the negative effects of freezing, high salinity,and drought on plants, as well as conferring other positive effects onplant phenotype; US2004/0148654 and WO 01/36596 where abscisic acid isaltered in plants resulting in improved plant phenotype such asincreased yield and/or increased tolerance to abiotic stress; WO00/006341, WO 04/090143, U.S. application Ser. Nos. 10/817,483 and09/545,334 where cytokinin expression is modified resulting in plantswith increased stress tolerance, such as drought tolerance, and/orincreased yield. Also see WO 02/02776, WO 03/052063, JP2002281975, U.S.Pat. No. 6,084,153, WO 01/64898, U.S. Pat. No. 6,177,275, and U.S. Pat.No. 6,107,547 (enhancement of nitrogen utilization and altered nitrogenresponsiveness). For ethylene alteration, see US2004/0128719,US2003/0166197 and WO 00/32761. For plant transcription factors ortranscriptional regulators of abiotic stress, see e.g. US2004/0098764 orUS2004/0078852.

Other genes and transcription factors that affect plant growth andagronomic traits such as yield, flowering, plant growth and/or plantstructure, can be introduced or introgressed into plants, see e.g. WO97/49811 (LHY), WO 98/56918 (ESD4), WO 97/10339 and U.S. Pat. No.6,573,430 (TFL), U.S. Pat. No. 6,713,663 (FT), WO 96/14414 (CON), WO96/38560, WO 01/21822 (VRN1), WO 00/44918 (VRN2), WO 99/49064 (GI), WO00/46358 (FRI), WO 97/29123, U.S. Pat. No. 6,794,560, U.S. Pat. No.6,307,126 (GAI), WO 99/09174 (D8 and Rht), and WO 04/076638 and WO04/031349 (transcription factors).

One may obtain soybean plants according to the present invention bydirectly growing the seed of OT21325816 or by any other means. A soybeanplant having all of the physiological and morphological characteristicsof OT21325816 can be obtained by any suitable means, including, but notlimited to, regenerating plants or plant parts from tissue culture orcuttings. The scope of the present invention is not limited by themethod by which the plant is obtained.

Tissue Cultures and Plants Regenerated Therefrom

The present invention provides a tissue culture of protoplasts orregenerable cells from a plant, or parts thereof, produced from soybeanvariety OT21325816, or a part thereof. In some embodiments, theprotoplasts or regenerable cells are derived from embryo, meristematiccell, leaf, pollen, ovule, cotyledon, hypocotyl, embryo, root, root tip,anther, pistil, pod, flower, shoot or stalk of soybean varietyOT21325816.

Tissue culture of various tissues of soybeans and regeneration of plantstherefrom is well known and widely published. For example, reference maybe had to Komatsuda, T. et al., “Genotype X Sucrose Interactions forSomatic Embryogenesis in Soybean,” Crop Sci. 31:333-337 (1991);Stephens, P. A. et al., “Agronomic Evaluation of Tissue-Culture-DerivedSoybean Plants,” Theor. Appl. Genet. (1991) 82:633-635; Komatsuda, T. etal., “Maturation and Germination of Somatic Embryos as Affected bySucrose and Plant Growth Regulators in Soybeans Glycine gracilis Skvortzand Glycine max (L.) Merr.,” Plant Cell, Tissue and Organ Culture,28:103-113 (1992); Dhir, S. et al., “Regeneration of Fertile Plants fromProtoplasts of Soybean (Glycine max L. Merr.): Genotypic Differences inCulture Response,” Plant Cell Reports (1992) 11:285-289; Pandey, P. etal., “Plant Regeneration from Leaf and Hypocotyl Explants of Glycinewightii (W. and A.) VERDC. var. longicauda,” Japan J. Breed. 42:1-5(1992); and Shetty, K., et al., “Stimulation of In Vitro ShootOrganogenesis in Glycine max (Merrill.) by Allantoin and Amides,” PlantScience 81:(1992) 245-251; as well as U.S. Pat. No. 5,024,944, issuedJun. 18, 1991 to Collins et al. and U.S. Pat. No. 5,008,200, issued Apr.16, 1991 to Ranch et al., the disclosures of which are herebyincorporated herein in their entirety by reference. Thus, another aspectof the present invention is to provide cells which upon growth anddifferentiation produce soybean plants having the physiological andmorphological characteristics of soybean variety OT21325816.

Soybean seeds, plants, and plant parts of variety OT21325816 may becleaned and/or treated. The resulting seeds, plants, or plant partsproduced by the cleaning and/or treating process(es) may exhibitenhanced yield characteristics. Enhanced yield characteristics caninclude one or more of the following: increased germination efficiencyunder normal and/or stress conditions, improved plant physiology, growthand/or development, such as water use efficiency, water retentionefficiency, improved nitrogen use, enhanced carbon assimilation,improved photosynthesis, and accelerated maturation, and improveddisease and/or pathogen tolerance. Yield characteristics can furthermoreinclude enhanced plant architecture (under stress and non-stressconditions), including but not limited to early flowering, floweringcontrol for hybrid seed production, seedling vigor, plant size,internode number and distance, root growth, seed size, fruit size, podsize, pod or ear number, seed number per pod or ear, seed mass, enhancedseed filling, reduced seed dispersal, reduced pod dehiscence and lodgingresistance. Further yield characteristics include seed composition, suchas carbohydrate content, protein content, oil content and composition,nutritional value, reduction in anti-nutritional compounds, improvedprocessability, and better storage stability.

Cleaning a seed or seed cleaning refers to the removal of impurities anddebris material from the harvested seed. Material to be removed from theseed includes but is not limited to soil, and plant waste, pebbles, weedseeds, broken soybean seeds, fungi, bacteria, insect material, includinginsect eggs, larvae, and parts thereof, and any other pests that existwith the harvested crop. The terms cleaning a seed or seed cleaning alsorefer to the removal of any debris or impurities such as low quality,infested, or infected seeds and seeds of different species that areforeign to the sample.

Treating a seed or applying a treatment to a seed refers to theapplication of a composition to a seed as a coating or otherwise. Thecomposition may be applied to the seed in a seed treatment at any timefrom harvesting of the seed to sowing of the seed. The composition maybe applied using methods including but not limited to mixing in acontainer, mechanical application, tumbling, spraying, misting, andimmersion. Thus, the composition may be applied as a powder, acrystalline, a ready-to-use, a slurry, a mist, and/or a soak. For ageneral discussion of techniques used to apply fungicides to seeds, see“Seed Treatment,” 2d ed., (1986), edited by K. A Jeffs (chapter 9),herein incorporated by reference in its entirety. The composition to beused as a seed treatment can comprise one or more of a pesticide, afungicide, an insecticide, a nematicide, an antimicrobial, an inoculant,a growth promoter, a polymer, a flow agent, a coating, or anycombination thereof. General classes or family of seed treatment agentsinclude triazoles, anilides, pyrazoles, carboxamides, succinatedehydrogenase inhibitors (SDHI), triazolinthiones, strobilurins, amides,and anthranilic diamides. In some examples, the seed treatment comprisestrifloxystrobin, azoxystrobin, metalaxyl, metalaxyl-m, mefenoxam,fludioxinil, imidacloprid, thiamethoxam, thiabendazole, ipconazole,penflufen, sedaxane, prothioconazole, picoxystrobin, penthiopyrad,pyraclastrobin, xemium, Rhizobia spp., Bradyrhizobium spp. (e.g., B.japonicum), Bacillus spp. (e.g., B. firmus, B. pumilus, B. subtilis),lipo-chitooligosaccharide, clothianidin, cyazapyr, rynaxapyr, abamectin,and any combination thereof. In some examples the seed treatmentcomprises trifloxystrobin, metalaxyl, imidacloprid, Bacillus spp., andany combination thereof. In some examples the seed treatment comprisespicoxystrobin, penthiopyrad, cyazapyr, ranaxapyr, and any combinationthereof. In some examples, the seed treatment improves seed germinationunder normal and/or stress environments, early stand count, vigor,yield, root formation, nodulation, and any combination thereof. In someexamples seed treatment reduces seed dust levels, insect damage,pathogen establishment and/or damage, plant virus infection and/ordamage, and any combination thereof.

Soybean Products

Soybean is useful not only as a seed for producing soybean plants, butalso has utility as a grain. The grain can be used as a food source forboth animals and humans. Soybean is widely used as a source of proteinfor animal feeds for poultry, swine and cattle. The soybean grain istherefore a commodity. The soybean commodity plant products include butare not limited to protein concentrate, protein isolate, soybean hulls,meal, flower, oil and the whole soybean itself.

During processing of whole soybeans, the fibrous hull is removed and theoil is extracted. The remaining soybean meal is a combination ofcarbohydrates and approximately 50% protein. For human or animalconsumption soybean meal is made into soybean flour that is processed toprotein concentrates used for meat extenders or specialty pet foods.Production of edible protein ingredients from soybean offers a healthyless expensive replacement for animal protein in meats as well asdairy-type products.

Accordingly, the present invention includes in some embodiments methodsfor producing a commodity plant product, which comprise obtaining seedof soybean variety OT21325816 and producing the commodity plant productsdisclosed above. The invention further comprises soybean commodity plantproducts derived from soybean variety OT21325816 seed according to thesemethods.

All publications, patents and patent applications referenced in thespecification are indicative of the level of those skilled in the art towhich this invention pertains. All such publications, patents and patentapplications are incorporated by reference herein to the same extent asif each was specifically and individually indicated to be incorporatedby reference herein.

The foregoing invention has been described in some detail by way ofillustration and example for purposes of clarity and understanding.However, it should be appreciated by those having ordinary skill in theart that certain changes and modifications such as single genemodifications and mutations, somoclonal variants, variant individualsselected from large populations of the plants of the instant novelvariety and the like may be practiced within the scope of the invention,as limited only by the scope of the appended claims, without departingfrom the true concept, spirit, and scope of the invention.

DEPOSIT INFORMATION

Seed from soybean variety OT21325816, disclosed above and recited in theappended claims, is irrevocably deposited under the Budapest Treaty withthe American Type Culture Collection (ATCC), 10801 University Boulevard,Manassas, Va. 20110 on Oct. 15, 2018 under the ATCC Accession No.PTA-125335. The seeds deposited are taken from seeds maintained byApplicant since prior to the filing date of this application. Access tothe ATCC deposit will be available during the pendency of theapplication to the Commissioner of Patents and Trademarks and personsdetermined by the Commissioner to be entitled thereto upon request. Uponallowance of any claims in the application, the Applicant will make thedeposit available to the public pursuant to 37 C.F.R. § 1.808 byaffording access to the deposit with the ATCC. Applicant has or willhave satisfied all of the requirements of 37 C.F.R. §§ 1.801-1.809. Thedeposit will be maintained in the ATCC depository for a period of 30years, or 5 years after the last request, or for the effective life ofthe patent, whichever is longer, and will be replaced if necessaryduring that period.

Soybean variety OT21325816 has excellent agronomic characteristicsincluding high yield potential relative to lines of similar maturity.Soybean variety OT21325816 is well-adapted to early maturity group II toearly maturity group III growing areas of Michigan, Ohio, Indiana, Iowa,Illinois, Minnesota, South Dakota, Nebraska and Wisconsin.

The breeding history of soybean variety OT21325816 is summarized inTable 1.

TABLE 1 Breeding Method for Cultivar OT21325816 Filial Generation MethodF0 cross between parents F0 cross between parents and an MG2 F0 crossbetween those parents and another MG3 F0 cross between those parents andanother MG3 F1 plant growout F2 population growout F3 progeny row,single-plant selection F4 plant-row yield trial F5 preliminary yieldtrial F6 purity reselection and seed increase F7 advanced yield trial F8seed increase F9 advanced yield trial

The variety description information (Table 2) provides a summary ofsoybean variety OT21325816 plant characteristics. Those of skill in theart will recognize that these are typical values that may vary due toenvironment and that other values that are substantially equivalent arewithin the scope of the invention.

TABLE 2 Physiological and Morphological Characteristics of CultivarOT21325816 Characteristic Value Relative Maturity 2.7 Maturity Date(days from January 1) 275 Hilum Color (Mature Seed) Buff Seed Coat Color(Mature Seed) Yellow Seed Coat Luster (Mature Hand Shelled Seed) DullCotyledon Color (Mature Seed) Yellow Emergence (1 to 9) 7.3 Canopy Width(1 to 9) 8.1 Growth Habit Indeterminate Plant Height (inches) 38.7Lodging (1 to 5) 2 Flower Color White Leaflet Shape Ovate PubescenceColor Gray Pod Wall Color Tan Shattering (1 to 9) 1 Seed Size (#Seeds/lb.) 3157 Resistance/Tolerance to Herbicides Roundup Ready(40-3-2) No Roundup Ready 2 (GM_A19788) No Sulfonylurea Tolerant Soybean(STS) No Enlist (E3) Event DAS-44406-6 Resistance/Tolerance to PestsSoybean Cyst Nematode (R, MR, MS, S) Race 3 MR Phytophthora Root RotResistance (single gene) Rps1C Phytophthora Tolerance (1 to 9) 2.25Brown Stem Rot (1 to 9) 2 Sclerotinia Stem Rot 4 Sudden Death Syndrome(1 to 9) 2.2 Tolerance to Environments Iron-Deficiency Chlorosis (1 to9) 3.3

In addition to the individual plant characteristics set forth in Table2, agronomic yield of soybean variety OT21325816 was evaluated. Table 3compares the yield and maturity difference of soybean variety OT21325816to those of other soybean varieties developed for a similarcrop-production region.

TABLE 3 Yield of Cultivar OT21325816 Compared to Selected CultivarsMaturity Years Paired Reps. Yield Yield Difference (#) Cultivart-test^(a) (#) (bu/ac) (%)^(b) (days) 1 OT21325816 NS 31 64.7 101 0AG2232 0.890 64.3 100 −3.8 1 OT21325816 NS 30 63.9 100 0 AG25X6 0.72064.6 101 −3.0 1 OT21325816 NS 23 57.7 101 0 AG2933 0.880 57.4 100 1.7 2OT21325816 NS 54 61.6 101 0 CR X282N 0.870 61.2 100 1.1 1 OT21325816 NS23 57.7 101 0 CR2902N 0.860 57.4 100 2.7 1 OT21325816 NS 31 64.7 105 0CS24R203 0.320 61.6 100 −6.9 ^(a)Thresholds for paired t-tests are nosignificant difference (NS) and significant at P < .05 (*), P < .01(**), and P < .001 (***). ^(b)Percentage yield relative to thelower-yielding cultivar in each two-way comparison.

What is claimed is:
 1. A plant or a seed of soybean variety OT21325816,representative seed of the variety having been deposited under ATCCAccession No. PTA-125335.
 2. A soybean plant, or part thereof, producedby growing the seed of claim
 1. 3. A method for producing treated seed,the method comprising applying a seed treatment to the seed of claim 1.4. A soybean seed obtained by introducing a transgene into the plant orseed of claim 1, wherein the soybean seed produces a soybean plantotherwise comprising all the physiological and morphologicalcharacteristics of soybean variety OT21325816 when grown under the sameenvironmental conditions.
 5. The seed of claim 4, wherein the transgeneconfers a trait selected from the group consisting of male sterility, asite-specific recombination site, abiotic stress tolerance, alteredphosphate, altered antioxidants, altered fatty acids, altered essentialamino acids, altered carbohydrates, herbicide resistance, insectresistance, and disease resistance.
 6. The seed of claim 4, wherein thetransgene is introduced by backcrossing or transformation.
 7. A soybeanplant produced by growing the seed of claim
 4. 8. A method fordeveloping a second soybean plant comprising applying plant breedingtechniques to the plant of claim 1, wherein application of thetechniques results in development of the second soybean plant.
 9. Amethod for producing soybean seed, the method comprising crossing twosoybean plants and harvesting the resultant soybean seed, wherein atleast one soybean plant is the soybean plant of claim
 1. 10. The F1soybean seed produced by the method of claim
 9. 11. A method comprisingisolating nucleic acids from the plant or seed of claim
 1. 12. A methodof producing a soybean plant comprising a locus conversion, the methodcomprising introducing a locus conversion into the plant of claim 1,wherein the locus conversion confers a trait selected from the groupconsisting of male sterility, a site-specific recombination site,abiotic stress tolerance, altered phosphate, altered antioxidants,altered fatty acids, altered essential amino acids, alteredcarbohydrates, herbicide resistance, insect resistance, and diseaseresistance.
 13. A soybean plant produced by the method of claim 12,wherein the soybean plant comprises the locus conversion and otherwisecomprises all of the physiological and morphological characteristics ofsoybean variety OT21325816 when grown under the same environmentalconditions.
 14. The soybean plant of claim 13, wherein the locusconversion comprises a transgene encoding a Bacillus thuringiensis (Bt)endotoxin.
 15. The method of claim 12, wherein the locus conversion isintroduced by backcrossing or transformation.
 16. A seed, plant, plantpart, or plant cell of soybean variety OT21325816, representative seedof the soybean variety OT21325816 having been deposited under ATCCAccession No. PTA-125335, wherein the seed, plant, plant part or plantcell of soybean variety OT21325816 further comprises a single locusconversion.
 17. A soybean plant expressing all the physiological andmorphological characteristics of the soybean plant of claim
 2. 18. Amethod of producing a commodity plant product comprising collecting thecommodity plant product from the plant of claim
 1. 19. The method ofclaim 18, wherein the commodity plant product is protein concentrate,protein isolate, grain, soybean hulls, meal, flour, or oil.
 20. Asoybean commodity plant product produced by the method of claim 18,wherein the commodity plant product comprises at least one cell ofsoybean variety OT21325816.